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PC-3

(ATCC? CRL-1435?)

Organism Homo sapiens, human

Tissue prostate; derived from metastatic site: bone

Product Format frozen

Morphology epithelial

Culture Properties adherent (The cells form clusters in soft agar and can be adapted to suspension growth)

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease grade IV, adenocarcinoma Age 62 years adult

Gender male Ethnicity Caucasian

Applications This cell line is a suitable transfection host.

Storage Conditions liquid nitrogen vapor phase

Karyotype The line is near-triploid with a modal number of 62 chromosomes. There are nearly 20 marker chromosomes commonly found in each cell; and normal N2, N3, N4, N5, N12, and N15 are not found. No normal Y chromosomes could be detected by Q-band analysis.

Derivation The PC-3 was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian

. Clinical Data 62 years adult Caucasian male The PC-3 was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian.

Antigen Expression HLA A1, A9

Genes Expressed HLA A1, A9

Tumorigenic Yes

Effects Yes, in semi-solid medium Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Comments The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities. omplete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning? T-75 flasks (catalog #430641) are recommended for subculturing this product.

2,Remove and discard culture medium.

2,Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3,Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37℃ to facilitate dispersal.

4,Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5,Add appropriate aliquots of the cell suspension to new culture vessels.

6,Incubate cultures at 37℃. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Cryopreservation Freeze medium:

Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase

Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37℃