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平臺(tái)編號(hào)：bio-72822
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國際編號(hào)：ATCC CCL-1.4
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細(xì)胞信息： Staphylococcus carnosus subsp. carnosus
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規(guī)格： Frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
A9
A9 (APRT and HPRT negative derivative of Strain L) 拉丁名
ATCCC CL-1.4 統(tǒng)一編號(hào)
Product category：Animal cells
Organism：Mus musculus, mouse
Classification：Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
Morphology：fibroblast
Tissue：Subcutaneous connective tissue; Adipose; Areolar
Disease：Normal
Applications：3D cell culture
Product format 提供形式 ：Frozen
Storage conditions：Vapor phase of liquid nitrogen
Growth properties：Adherent
Derivation：The A9 cell line was isolated by J.W. Littlefield from wild-type Strain L cells.
Age：100 days
Gender：Male
Strain：C3H/An
Karyotype：modal number = 52; range = 46 to 54; This is a hyperdiploid cell line with 24 to 26 marker chromosomes common to all cells., Nine markers (M1 - M9) are common to L-M(TK-) (see ATCC CCL-1.3) and 16 to 17 are found only in A9. The latter markers include Rb(3.5), Rb(4.15), Rb(5.5), T(8;19), Rb(9.17) and 11 to 12 others., Structurally normal chromosomes 4, 15, 16, 17, 18 and X were absent. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Tumorigenic：Yes; Yes, in nude mice
Antigen expression：H-2k
Comments：1 The cells are sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT).
2 The cells are resistant to 8-azaguanine (0.003 mg/mL) and 6-thioguanine (0.1 mM).
Unpacking and storage instructions
Check all containers for leakage or breakage.
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below --130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium：Dulbecco's modified Eagle's medium with 4.5g/L glucose, 90%; fetal bovine serum, 10%
Temperature：37°C
Atmosphere：95% Air, 5% CO2
