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Hepa 1-6 [Hepa1-6]

(ATCC? CRL-1830?)

Organism Mus musculus, mouse

Tissue liver

Product Format frozen Morphology epithelial Culture Properties adherent

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease hepatoma

Strain C57L

Applications 用途 This line is a suitable transfection host.

Storage Conditions liquid nitrogen vapor phaseDerivation This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.

Genes Expressed albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase

Cellular Products albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase

Comments 注釋 Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

1,Remove and discard culture medium.

2,Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3,Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37℃ to facilitate dispersal.

4,Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5,Add appropriate aliquots of the cell suspension to new culture vessels.

6,Incubate cultures at 37℃.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Two to three times weekly

Cryopreservation Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase

Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37℃

Growth Conditions: The cells can also be propagated in a serum free medium consisting of 3 parts Dulbecco's modified Eagle's medium, 1 part Waymouth's MAB 87/3 medium and 30 nM selenium.