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C-33 A [c-33a]  拉丁名

(ATCC? HTB-31?) 統(tǒng)一編號

Organism Homo sapiens, human

Tissue cervix

Cell Type Epithelial, Retinoblastoma

Product Format 提供形式 frozen

Morphology epithelial

Culture Properties adherent

Biosafety Level 生物安全等級 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease carcinoma

Age 66 years adult

Gender female

Ethnicity Caucasian

Storage Conditions 保藏條件 liquid nitrogen vapor phase 

Karyotype This a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 70% of cells examined.Polyploid cells occurred at 8.6%. Seven marker chromosomes were consistently detected per pseudodiploid cell.

They are: t(1q17q), t(1p21q), del(18) (q21.3), der(1)t(1;17) (p16;q21.3) and three others. Several other markers were also found but they occurred only once in 15 metaphases analyzed. Neither DMs nor HSRs were detected. Structurally normal N1 was absent. Generally there are two X chromosomes in each cell.

Clinical Data 66 years adult

Caucasian female

Oncogene p53 +; pRB +

Genes Expressed Oncogenes: p53 +; pRB +

Tumorigenic Yes

Effects Yes, in nude mice; forms undifferentiated carcinoma

Comments 注釋 The line exhibited a hypodiploid karyotype initially, and an epithelial morphology.

Karyological instability was observed with continued passage. The retinoblastoma protein (pRB) is present but abnormal in size.

Expression of p53 is elevated, and there is a point mutation at codon 273 resulting in a Arg -> Cys substitution.

The cells are negative for human papillomavirus DNA and RNA. The C-33 A cell line is one of a series of lines (see also ATCC CRL-1594 and ATCC CRL-1595) derived by N. Auersperg from cervical cancer biopsies.

Complete Growth Medium 完全培養(yǎng)基 The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution.

Allow the flask to sit at room temperature (or at 37℃) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium 培養(yǎng)基 Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium 冷凍培養(yǎng)基 : Culture medium, 95%; DMSO, 5%

Storage temperature 保藏溫度 : liquid nitrogen vapor phase

Culture Conditions 培養(yǎng)條件

Temperature 培養(yǎng)溫度 : 37℃

Atmosphere 需氧情況 : air, 95%; carbon dioxide (CO2), 5%

STR Profile Amelogenin: X

CSF1PO: 12

D13S317: 13

D16S539: 13,14

D5S818: 11,12

D7S820: 10

THO1: 7,8

TPOX: 9

vWA: 18,20

Isoenzymes AK-1, 1

ES-D, 1

G6PD, B GLO-I, 2

Me-2, 2

PGM1, 1

PGM3, 1

Name of Depositor 寄存人 N Auersperg

Passage History The C-33 A cell line is one of a series of lines (see also ATCC CRL-1594 and ATCC CRL-1595) derived by N.

Auersperg from cervical cancer biopsies. The line exhibited a hypodiploid karyotype initially, and an epithelial morphology.

Karyological instability was observed with continued passage. The retinoblastoma protein (pRB) is present but abnormal in size.

Expression of p53 is elevated, and there is a point mutation at codon 273 resulting in a Arg -> Cys substitution. The cells are negative for human papillomavirus DNA and RNA.

References 參考文獻(xiàn) J. Natl. Cancer Inst. 32: 135-148, 1964.

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

Kovelman R, et al. Enhanced transcriptional activation by E2 proteins from the oncogenic human papillomaviruses. J. Virol. 70: 7549-7560, 1996. PubMed: 8892874