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P388D1 [P388 D1] 

(ATCC? CCL-46?)

Organism Mus musculus

Cell Type monocyte; macrophage

Product Format frozen

Morphology lymphoblast

Culture Properties suspension

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma Strain DBA/2

Applications transfection host

Storage Conditions liquid nitrogen vapor phase

Tumorigenic Yes

Effects Yes, in nude mice

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Comments A subclone of this line [see ATCC TIB-63, P388D1(IL-1)] produces high levels of interleukin-1 (IL-1, interleukin 1). Tested and found negative for ectromelia virus (mousepox)

.Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 5 x 105 viable cells/mL. A maximum of 6 x 106 viable cells/mL is obtainable. Medium Renewal: Every 2 to 3 days

Cryopreservation Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0℃

Name of Depositor EM Jensen

References Dawe CJ, Potter M. Morphologic and biologic progression of a lymphoid neoplasm of the mouse in vivo and in vitro. Am. J. Pathol. 33: 603, 1957 Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

Cross References Nucleotide (GenBank) : U88624 Mus musculus 85 kDa calcium-independent phospholipase A2 mRNA, complete cds.