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HL-60 (ATCC? CCL-240?)

Organism Homo sapiens, human

Tissue peripheral blood

Cell Type promyeloblast

Product Format frozen

Morphology myeloblastic

Culture Properties suspension

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease acute promyelocytic leukemia Age 36 years

Gender female Ethnicity Caucasian

Applications This cell line is a suitable transfection host.

Storage Conditions liquid nitrogen vapor phaseKaryotype The stemline chromosome number is pseudodiploid with the 2S component occurring at 6.2%. Five markers (M2 through M6) were common to most S metaphases. DM's, which varied in numbers per cell, occurred in all metaphases karyotyped. HSR chromosomes were not detected.

Derivation HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia.

Clinical Data 36 years Caucasian female

Receptor Expression complement, expressed Ref Fc, expressed Ref

Oncogene myc + Genes

Expressed tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid,myc +

Cellular Products tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid Tumorigenic Yes Effects Yes, in nude mice (subcutaneous myeloid tumors) Yes, in semi-solid media

Comments HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli

The line is positive for myc oncogene expression.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 105 viable cells/mL. Do not allow cell concentration to exceed 1 X 106 cells/mL. Corning? T-75 flasks (catalog #431464) are recommended for subculturing this product. Interval: Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation Freeze medium:

Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37℃