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SYF（小鼠胚胎成纖維細胞（SV40））
產(chǎn)品類別：小鼠細胞系
生長特性：貼壁生長
細胞形態(tài)：Fibroblast
凍存條件：90%FBS+10%DMSO
Synonyms: SYF(小鼠胚胎成纖維細胞（SV40）)
Background: SYF (deficient for Src, Yes, and Fyn) cells were generated in 1997 from mouse embryos harboring functional null mutations in both alleles of the Src family protein tyrosine kinases, Src, Yes, and Fyn.
The cells were immortalized before passage 2 by infection with a retroviral vector transducing the SV40 large T antigen. SYF cells also carry a gene for neomycin resistance.
Species: Mus musculus, mouse
Tissue: embryo
Gender: 9 days gestation
Morphology: fibroblast
Growth Mode: adherent
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium
Cryopreservation medium: 90%FBS+10%DMSO
Oncogene: src -; yes -; fyn -
Comments: Src family kinases have been implicated as key regulators of ligand-induced cellular responses including proliferation, survival, adhesion and migration.
This is the first cell line isolated that lacks all three ubiquitously expressed Src family members.
SYF cells display reduced mobility in wound healing and haptotaxis assays but not in platelet derived growth factor (PDGF) induced chemotaxis.