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運輸方式：凍存運輸
細胞類型：B淋巴細胞
是否是腫瘤細胞：1
物種來源：人
ATCC Number：CRL-8033-1?
生長狀態(tài)：懸浮生長
數(shù)量：大量
細胞形態(tài)：淋巴樣
免疫類型：IgE; lambda light chain
規(guī)格：ea Designations: SKO-007
Depositors: ?H Kaplan, Stanford University Board of Trustees
Isotype: IgE; lambda light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast


Source: Cell Type: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:HLA A2, A3, B7, Bw60, Bw6, Cw3
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 12
D16S539: 10
D5S818: 11,12
D7S820: 11,12
THO1: 5,7
TPOX: 8
vWA: 17
Comments:This line was derived from the U266 myeloma cell line of Nilsson (Clin. Exp. Immunol. 7:477-489, 1970) by selecting for resistance to 8-azaguanine;the cells cells are HAT sensitive.
This is a batch of SKO-007 (formerly available as ATCC CRL-8033) that was cured at the ATCC of a mycoplasma contamination.
The J3 clone of SKO-007 is available also (ATCC CRL-8033-2 ).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.02 mg/ml 6-thioguanine, 1 mM sodium pyruvate and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Temperature: 37.0℃
Subculturing: Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products:parental cell line:ATCC CRL-8033
References: 1214: Olsson L, Kaplan HS. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc. Natl. Acad. Sci. USA 77: 5429-5431, 1980. PubMed: 6159646