是否是腫瘤細(xì)胞：1
物種來(lái)源：人
年限：43 years
ATCC Number：CCL-250?
相關(guān)疾?。捍竽c癌
生長(zhǎng)狀態(tài)：貼壁，多細(xì)胞聚集
細(xì)胞形態(tài)：上皮樣
數(shù)量：大量
器官來(lái)源：盲腸
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：100 ul Designations: SNU-C2B
Depositors: ?AF Gazdar
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:loosely adherent, multicell aggregates
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: cecum
Disease: colorectal carcinoma
Cellular Products:carcinoembryonic antigen (CEA), 24 ng/ml per 10 exp6 cells per 10 days
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Receptors:epidermal growth factor (EGF)
Tumorigenic:Yes
Antigen Expression:Blood type A; Rh+
DNA Profile (STR):Amelogenin: X
CSF1PO: 11, 14
D13S317: 9, 10, 11
D16S539: 8, 9
D5S818: 8, 9
D7S820: 13, 16
THO1: 9
TPOX: 8
vWA: 15, 18, 19
Cytogenetic Analysis:modal number = 46; range = 45 to 48.
This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring at 70% with polyploidy at 16.4%. The del (3) (p23), t(4,?), t(8;?) (p22), del (9) (p13), del (11) (p13), t(1;8) (p13;p24), t(1;?) (q11;?) and 2 other marker chromosomes are common to all metaphases examined. Other structurally altered chromosomes, if any, occur only sporadically. The t(1;8) and t(1;?) are not found in CCL-250.1. Normal N8 and N11 are absent. The X chromosomes are two copies per cell. The Y chromosome is not detected by Q-banding.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1
Age: 43 years
Gender: female
Ethnicity: Asian
Comments:This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The line was obtained from a fourth passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy (see also ATCC CCL-250.1 which was derived from the third nude mouse passage).
The cells have a high concentration of receptors for EGF.
Note that this line has lost both chromosomes 8 and 11 while SNU-C2A (ATCC CCL-250.1 ) has lost only chromosome 11.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249
