生長狀態(tài)：漂浮生長
器官來源：盲腸
是否是腫瘤細胞：1
物種來源：人
數量：大量
細胞形態(tài)：上皮樣
年限：55 years
ATCC Number：CCL-253?
相關疾病：大腸癌
運輸方式：凍存運輸
規(guī)格：40 ul Designations: NCI-H508 [H508]
Depositors: ?AF Gazdar
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:floating aggregates of round cells with some attached cells
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: cecum
Disease: colorectal adenocarcinoma
Cellular Products:carcinoembryonic antigen (CEA), 1709 ng/ml per 10 exp6 cells per 10 days
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.
Tumorigenic:Yes
Antigen Expression:Blood type A; Rh+; CA19-9 antigen
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 12
D7S820: 8,12
THO1: 9,9.3
TPOX: 8,11
vWA: 15,16
Cytogenetic Analysis:modal number = 102; range = 71 to 131; most cells have DM's
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 55 years
Gender: male
Ethnicity: Caucasian
Comments:This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.
The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for two minutes. Remove the trypsin and let the flask sit at room temperature for 10 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249
