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細(xì)胞形態(tài)：上皮樣
ATCC Number：CRL-5813
相關(guān)疾?。浩渌膊?br /> 細(xì)胞類型：其他細(xì)胞類型
年限：stage E
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：前列腺
數(shù)量：大量
生長(zhǎng)狀態(tài)：漂浮生長(zhǎng)
規(guī)格：0.1ml                                                                                                                                                                                                                                                                                                                                                          Designations: NCI-H660
         Depositors:                  ?AF Gazdar, JD Minna
         Biosafety Level:1
         Shipped:        frozen
         Medium & Serum: See Propagation          Growth        Properties:floating and attached clusters
         Organism:          Homo sapiens
         Morphology:epithelial
         

         Source:          Organ: prostate
         Tumor Stage: stage E
         Disease: small cell carcinoma
         Derived from metastatic site: lymph node
         Cell Type: epithelial neuroendocrine;
         Permits/Forms:In addition to the MTA          mentioned above, other ATCC            and/or regulatory permits  may be required for the          transfer of this ATCC material. Anyone purchasing ATCC          material is ultimately responsible for obtaining the permits.          Please click here  for information regarding          the specific requirements for shipment to your location.
             Restrictions:The line is available with the          following restrictions: 1. This cell line was deposited at the          ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for          research purposes only. Neither the  cell line nor products          derived from it may be sold or used for commercial purposes.          Nor can the cells be distributed to third parties for purposes          of sale, or producing for sale, cells or their products. The          cells are provided as  service to the research community. They          are provided without warranty of merchantability or fitness          for a particular purpose or any other warranty, expressed or          implied. 2. Any proposed commercial use of the these cells, or          their  products must first be negotiated with the University          of Texas Southwestern Medical Center at Dallas, 5323 Harry          Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056,          FAX (214) 688-7233.
         Receptors:atrial natriuretic peptide (ANP), expressed
         Reverse        Transcript:N
         DNA          Profile (STR):Amelogenin: X
CSF1PO: 12
         D13S317: 8
D16S539: 11
D5S818: 11
D7S820:          8,10
THO1: 7,9.3
TPOX: 8
vWA: 17
         Age:        63 years adult
         Gender:        male
         Ethnicity:        Caucasian
         HeLa          Markers: N
         Comments:While NCI-H660 has the appearance and          many of the properties of small cell carcinoma, there are also          differences. NCI-H660 expresses elevated levels of L-dopa          carboxylase and bombesin-like immunoreactivity. The cells          express functional atrial natriuretic peptide (ANP) receptors,          but ANP addition produces no detectable change in the cells'          growth pattern.
         Propagation:                   ATCC complete growth medium:  HITES medium          supplemented with 5% fetal bovine serum

1. The base medium for this cell line is ATCC-formulated            RPMI-1640 Medium, Catalog No.30-2001. To make the complete            growth medium, add the following components to the base              medium

2. 0.005 mg/ml Insulin

3. 0.01 mg/ml Transferrin

4. 30nM Sodium selenite (final conc.)

5. 10 nM Hydrocortisone (final conc.)

6. 10 nM beta-estradiol (final conc.)

7. extra 2mM L-glutamine (for final conc. of 4 mM)

8. 5% fetal bovine serum (final conc.)

         Atmosphere: air, 95%; carbon dioxide (CO2),          5%
         Temperature: 37.0°C
         Subculturing:                  Protocol: Add medium as the cell density          increases. Shake flask to dislodge attached cells. Single          cells are often dead and clusters are viable. Subculture when          there are numerous, healthy-appearing clusters present in          suspension.
         Medium Renewal: Every 2 to 3 days
         Preservation:                  Freeze medium:  Complete growth medium;90% ;          DMSO, 10%.
         Storage temperature: liquid nitrogen vapor phase
         Related        Products:Recommended medium (without the          additional supplements or serum described under ATCC Medium):          ATCC 30-2001
recommended serum:ATCC 30-2020
L-Glutamine Solution, 200          mM:ATCC 30-2214
         References:           22725: Lai SL, et al. Molecular genetic          characterization of neuroendocrine lung cancer cell lines.          Anticancer Res. 15: 225-232, 1995. PubMed: 7762988
          23056: Carney DN, et al. Establishment          and identification of small cell lung cancer cell lines having          classic and variant features.  Cancer Res. 45: 2913-2923,          1985. PubMed: 2985257
          23227: Johnson BE, et al. Retention of          chromosome 3 in extrapulmonary small cell cancer shown by          molecular and cytogenetic studies.  J. Natl. Cancer Inst. 81:          1223-1228, 1989. PubMed: 2569043