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年限：48 years
運輸方式：凍存運輸
生長狀態(tài)：懸浮生長
數(shù)量：大量
細(xì)胞形態(tài)：上皮樣
器官來源：腎上腺
ATCC Number：CRL-10296?
相關(guān)疾?。浩渌膊?br /> 組織來源：cortex
是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：0.1ml Designations: NCI-H295 [H295]
Depositors: ?AF Gazdar
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, with some loosely adherent cells, suspension with some loosely attached cells
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: adrenal gland
Tissue: cortex
Disease: adrenocortical carcinoma
Cellular Products:steroid hormones
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 13
D16S539: 11
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8
vWA: 17,18
Cytogenetic Analysis:modal number = 62; range = 55 to 64; hypertriploid. Twenty-three marker chromosomes were common to most cells: der(2)t(2;18)(p25;p11), del(3)(q21), der(10)t(10;13)(p15;q11), der(7)t(7;7)(q36;p13), del(7)(q11),; der(8)t(7;8)(p13;p23), del(8)(p12), del(9)(p22), del(9)(p13), del(11)(p12), del(12)(p12), t(13q18q) and 11 others. The del(9)(p13) was paired; normal N13 was absent. N15 had three copies and N22 had four copies.; A total of 65 marker chromosomes were detected in 38 metaphases examined.; A minor subline that occurred in about 16% of the cells was characterized by the presence of del(M5)(7p11) replacing M5, by the single N17 (instead of paired N17) and by the presence of an additional M42.
Age: 48 years
Gender: female
Ethnicity: Black
Propagation: ATCC complete growth medium: HITES medium supplemented with 2% fetal bovine serum

HITES medium with 2% fetal bovine serum is formulated at the ATCC as follows:
• Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006)
• Insulin 0.005 mg/ml
• Transferrin 0.01 mg/ml
• Sodium selenite 30 nM
• Hydrocortisone 10 nM
• beta-estradiol 10 nM
• HEPES 10 mM
• L-glutamine 2 mM (in addition to that in the base medium)
• fetal bovine serum 2%
  Temperature: 37.0°C
  Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Cells grow as floating aggregates of round cells with some loosely attached cells. Shake the flask to dislodge the cells and transfer to a new flask.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with an additional 8% fetal bovine serum and 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 23082: Gazdar AF, et al. Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis. Cancer Res. 50: 5488-5496, 1990. PubMed: 2386954