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平臺編號：bio-69517
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國際編號：HTB-137?
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細胞信息： Hs 695T
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規(guī)格：Frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Hs 695T
數(shù)量：大量
運輸方式：凍存運輸
細胞形態(tài)：上皮樣
器官來源：皮膚
是否是腫瘤細胞：1
物種來源：人
生長狀態(tài)：貼壁生長
年限：26 years adult
ATCC Number：HTB-137?
相關疾病：其他疾病
規(guī)格：48T Designations: Hs 695T
Depositors: ?RB Owens
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: skin
Disease: melanoma, amelanotic
Derived from metastatic site: lymph node
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1973
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11
D13S317: 12
D16S539: 9,13
D5S818: 9
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 18
Cytogenetic Analysis:(P19-40) mode = 52; Y chromosome present
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 1
Age: 26 years adult
Gender: male
Ethnicity: Caucasian
Comments:Hs 695T was isolated in 1973 from the lymph node metastasis of an amelanotic melanoma by A. Creasey et al. The cells had a doubling time of approximately 48 hours at passages 19 through 40 and grew moderately in semi-solid medium.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22658: Creasey AA, et al. Biological properties of human melanoma cells in culture. In Vitro 15: 342-350, 1979. PubMed: 478563
運輸方式：凍存運輸
細胞形態(tài)：上皮樣
器官來源：皮膚
是否是腫瘤細胞：1
物種來源：人
生長狀態(tài)：貼壁生長
年限：26 years adult
ATCC Number：HTB-137?
相關疾病：其他疾病
規(guī)格：48T Designations: Hs 695T
Depositors: ?RB Owens
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: skin
Disease: melanoma, amelanotic
Derived from metastatic site: lymph node
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1973
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11
D13S317: 12
D16S539: 9,13
D5S818: 9
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 18
Cytogenetic Analysis:(P19-40) mode = 52; Y chromosome present
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 1
Age: 26 years adult
Gender: male
Ethnicity: Caucasian
Comments:Hs 695T was isolated in 1973 from the lymph node metastasis of an amelanotic melanoma by A. Creasey et al. The cells had a doubling time of approximately 48 hours at passages 19 through 40 and grew moderately in semi-solid medium.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22658: Creasey AA, et al. Biological properties of human melanoma cells in culture. In Vitro 15: 342-350, 1979. PubMed: 478563
