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平臺編號：bio-69468
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國際編號：CRL-8083?
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細胞信息： MC/CAR
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
MC/CAR
ATCC Number：CRL-8083?
相關疾?。憾喟l(fā)性骨髓瘤
器官來源：外周血
生長狀態(tài)：懸浮生長
數(shù)量：大量
運輸方式：凍存運輸
細胞類型：B淋巴細胞
是否是腫瘤細胞：1
物種來源：人
細胞形態(tài)：淋巴樣
免疫類型：IgG1 (cytoplasmic); kappa light chain
年限：81 years Designations: MC/CAR
Depositors: ?Research Corporation
Isotype: IgG1 (cytoplasmic); kappa light chain
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: plasmacytoma; myeloma
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:complement (C3b)
Tumorigenic:Yes
Antigen Expression:HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,13
D16S539: 11,12
D5S818: 11,14
D7S820: 9,13
THO1: 6,9
TPOX: 8
vWA: 17,18
Cytogenetic Analysis:normal male diploid
Isoenzymes: ADA, 1
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1
PGM3, 1
Age: 81 years
Gender: male
Ethnicity: Caucasian
Comments:Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270 ]
The cells produce, but do not secrete, immunoglobulin.
No surface immunoglobulin.
8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.
Can be used as a human fusion line.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3535: Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984
43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375
43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762
58062: Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719
相關疾?。憾喟l(fā)性骨髓瘤
器官來源：外周血
生長狀態(tài)：懸浮生長
數(shù)量：大量
運輸方式：凍存運輸
細胞類型：B淋巴細胞
是否是腫瘤細胞：1
物種來源：人
細胞形態(tài)：淋巴樣
免疫類型：IgG1 (cytoplasmic); kappa light chain
年限：81 years Designations: MC/CAR
Depositors: ?Research Corporation
Isotype: IgG1 (cytoplasmic); kappa light chain
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: plasmacytoma; myeloma
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:complement (C3b)
Tumorigenic:Yes
Antigen Expression:HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,13
D16S539: 11,12
D5S818: 11,14
D7S820: 9,13
THO1: 6,9
TPOX: 8
vWA: 17,18
Cytogenetic Analysis:normal male diploid
Isoenzymes: ADA, 1
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1
PGM3, 1
Age: 81 years
Gender: male
Ethnicity: Caucasian
Comments:Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270 ]
The cells produce, but do not secrete, immunoglobulin.
No surface immunoglobulin.
8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.
Can be used as a human fusion line.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3535: Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984
43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375
43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762
58062: Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719
