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平臺編號：bio-69463
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國際編號：HTB-152?
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細(xì)胞信息： Hs 913T
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Hs 913T
生長狀態(tài)：貼壁生長
ATCC Number：HTB-152?
相關(guān)疾?。豪w維肉瘤
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：成纖維樣
是否是腫瘤細(xì)胞：1
物種來源：人
年限：51 years Designations: Hs 913T
Depositors: ?RB Owens
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Disease: fibrosarcoma
Derived from metastatic site: lung
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 10
D7S820: 10
THO1: 8,9.3
TPOX: 9,11
vWA: 17,18
Cytogenetic Analysis:modal number = 111 to 113; range = 95 to 120; large submetacentric and double minute (DM) markers
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 2
PGM3, 2
Age: 51 years
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; DMSO, 5%
ATCC Number：HTB-152?
相關(guān)疾?。豪w維肉瘤
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：成纖維樣
是否是腫瘤細(xì)胞：1
物種來源：人
年限：51 years Designations: Hs 913T
Depositors: ?RB Owens
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Disease: fibrosarcoma
Derived from metastatic site: lung
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 10
D7S820: 10
THO1: 8,9.3
TPOX: 9,11
vWA: 17,18
Cytogenetic Analysis:modal number = 111 to 113; range = 95 to 120; large submetacentric and double minute (DM) markers
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 2
PGM3, 2
Age: 51 years
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; DMSO, 5%
