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平臺編號：bio-69451
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國際編號：HTB-53?
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細胞信息： A-427
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規(guī)格：Frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
A-427
是否是腫瘤細胞：1
物種來源：人
運輸方式：凍存運輸
年限：52 years
器官來源：肺
細胞形態(tài)：上皮樣
ATCC Number：HTB-53?
相關疾?。耗[瘤
數(shù)量：大量
生長狀態(tài)：貼壁生長
規(guī)格：10 μg Designations: A-427
Depositors: ?DJ Giard
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: lung
Disease: carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11,13
D5S818: 12
D7S820: 8,12
THO1: 9
TPOX: 8,11
vWA: 17
Cytogenetic Analysis:at passage 60, hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker
Isoenzymes: AK-1, 2
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1-2
PGM3, 1
Age: 52 years
Gender: male
Ethnicity: Caucasian
Comments:The A-427 line was derived by D.J. Giard, as indicated in the description for ATCC HTB-41 .
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: 1:2 to 1:6
Medium Renewal: Twice per week
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
物種來源：人
運輸方式：凍存運輸
年限：52 years
器官來源：肺
細胞形態(tài)：上皮樣
ATCC Number：HTB-53?
相關疾?。耗[瘤
數(shù)量：大量
生長狀態(tài)：貼壁生長
規(guī)格：10 μg Designations: A-427
Depositors: ?DJ Giard
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: lung
Disease: carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11,13
D5S818: 12
D7S820: 8,12
THO1: 9
TPOX: 8,11
vWA: 17
Cytogenetic Analysis:at passage 60, hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker
Isoenzymes: AK-1, 2
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1-2
PGM3, 1
Age: 52 years
Gender: male
Ethnicity: Caucasian
Comments:The A-427 line was derived by D.J. Giard, as indicated in the description for ATCC HTB-41 .
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: 1:2 to 1:6
Medium Renewal: Twice per week
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
