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平臺編號：bio-69450
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國際編號：CRL-8644?
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細(xì)胞信息： HuNS1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
HuNS1
數(shù)量：大量
生長狀態(tài)：懸浮生長
ATCC Number：CRL-8644?
相關(guān)疾?。浩渌膊?br /> 細(xì)胞形態(tài)：淋巴樣
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：淋巴母細(xì)胞
是否是腫瘤細(xì)胞：1
物種來源：人 Designations: HuNS1
Depositors: ?Medical College of Wisconsin Research Foundation, Inc.
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: myeloma
Cell Type: lymphoblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 10,12
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 16,19
Gender: male
Comments:This line was derived from the GM4672 cell line (Human Genetic Mutant Cell Repository, Coriell Institute for Medical Research) following treatment with ethylmethanesulfonate (EMS).
The cells are HAT sensitive, do not secrete immunoglobulin and are negative for Epstein-Barr virus.
The line is suitable for use as a fusion partner for human B cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 4.5g/L glucose, 10 mM HEPES, 1 mM sodium pyruvate, 1.5 g/L sodium bicarbonate, 0.02 mM 2-amino-6-methylmercaptopurine, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: Complete Culture Medium, 95%; DMSO, 5%.
Storage temperature: liquid nitrogen vapor phase
References: 3864: Winkelhake JL. Myelomas for producing human/human hybridomas. US Patent 4,720,459 dated Jan 19 1988
生長狀態(tài)：懸浮生長
ATCC Number：CRL-8644?
相關(guān)疾?。浩渌膊?br /> 細(xì)胞形態(tài)：淋巴樣
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：淋巴母細(xì)胞
是否是腫瘤細(xì)胞：1
物種來源：人 Designations: HuNS1
Depositors: ?Medical College of Wisconsin Research Foundation, Inc.
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: myeloma
Cell Type: lymphoblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 10,12
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 16,19
Gender: male
Comments:This line was derived from the GM4672 cell line (Human Genetic Mutant Cell Repository, Coriell Institute for Medical Research) following treatment with ethylmethanesulfonate (EMS).
The cells are HAT sensitive, do not secrete immunoglobulin and are negative for Epstein-Barr virus.
The line is suitable for use as a fusion partner for human B cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 4.5g/L glucose, 10 mM HEPES, 1 mM sodium pyruvate, 1.5 g/L sodium bicarbonate, 0.02 mM 2-amino-6-methylmercaptopurine, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: Complete Culture Medium, 95%; DMSO, 5%.
Storage temperature: liquid nitrogen vapor phase
References: 3864: Winkelhake JL. Myelomas for producing human/human hybridomas. US Patent 4,720,459 dated Jan 19 1988
