生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：1
物種來源：人
年限：56 years
ATCC Number：CRL-1976?
相關(guān)疾?。浩渌膊?br />
細(xì)胞形態(tài)：成纖維樣
器官來源：子宮
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸 Designations: MES-SA
Depositors: ?BI Sikic
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: uterus
Disease: uterine sarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1980
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 11,12
D5S818: 13
D7S820: 7,11
THO1: 6
TPOX: 8,11
vWA: 18
Cytogenetic Analysis:This is a hypo-diploid human cell line with the modal chromosome number of 45 in 48% of cells examined. The rate of polyploidy was 2.7%. There were five or six marker chromosomes present in each cell. Whereas t(5q6p), 10q+, and 14q+ markers were common in all cells examined, the chromosome combination of either double copy N21 lacking M5 (66.7%) or t(21qter--->C--->?)(M5)/single copy N21/minute metacentric (33.3%) distinguishes the two coexisting clones. Both X chromosomes were normal.
Age: 56 years
Gender: female
Ethnicity: Caucasian
Comments:The MES-SA cell line was established from a surgical tumor specimen obtained at the time of hysterectomy.
The tumor was described as a poorly differentiated uterine sarcoma.
Initially, the cells were grown in soft agar, and later they were transferred to muliwell plates.
The nonepithelial origin of the cells was supported by ultrastructural studies and the absence of staining for mucin.
The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin.
They are resistant to vinblastine, dacarbazine, cisplatin, melphalan, vincristine, methotrexate and etoposide.
The multiple drug resistant cell line MES-SA/Dx5 (ATCC CRL-1977 ) was established from MES-SA cells which were grown in the presence of doxorubicin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Growth Conditions: Newborn calf serum may be substituted for fetal bovine serum.
Subculturing: Protocol: Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 22 to 24 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
derivative:ATCC CRL-1977
derivative:ATCC CRL-2274
References: 23048: Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344
