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平臺編號：bio-69438
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國際編號：CRL-1579?
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細胞信息： C32TG
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規(guī)格：Frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
C32TG
生長狀態(tài)：貼壁生長
運輸方式：凍存運輸
器官來源：皮膚
數(shù)量：大量
是否是腫瘤細胞：1
物種來源：人
ATCC Number：CRL-1579?
相關(guān)疾?。浩渌膊?br /> 年限：53 adult Designations: C32TG [C32-r16TG]
Depositors: ?TR Chen
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:
Source: Organ: skin
Disease: melanoma, amelanotic
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 12
D7S820: 9,11
THO1: 9.3
TPOX: 9
vWA: 14,19
Age: 53 adult
Gender: male
Ethnicity: Caucasian
Comments:The cells are resistant to 6-thioguanine and sensitive to HAT medium.
This line was derived from C32 (ATCC CRL-1585 ) by selection for 6-thioquanune resistance.
They are deficient in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-).
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, 90%; fetal bovine serum, 10%; 0.050 mM 6-thioguanine
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution several minutes until cells detach. Add fresh medium and centrifuge. Resuspend in fresh medium and dispense into new flasks.
Related Products:derivative:ATCC CRL-1585
References: 1082: Chen TR. Chromosome changes in 6-TG-resistant mutant strains derived from a karyotypically stable human line, C32. Cytogenet. Cell Genet. 35: 181-189, 1983. PubMed: 6861522
26091: Chen TR. Evolution in vitro of stemlines with minimal karyotypic deviations in a human heteroploid cell line. J. Natl. Cancer Inst. 61: 277-284, 1978. PubMed: 277716
26092: Chen TR, Shaw MW. Stable chromosome changes in human malignant melanoma. Cancer Res. 33: 2042-2047, 1973. PubMed: 4725368
運輸方式：凍存運輸
器官來源：皮膚
數(shù)量：大量
是否是腫瘤細胞：1
物種來源：人
ATCC Number：CRL-1579?
相關(guān)疾?。浩渌膊?br /> 年限：53 adult Designations: C32TG [C32-r16TG]
Depositors: ?TR Chen
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:
Source: Organ: skin
Disease: melanoma, amelanotic
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 12
D7S820: 9,11
THO1: 9.3
TPOX: 9
vWA: 14,19
Age: 53 adult
Gender: male
Ethnicity: Caucasian
Comments:The cells are resistant to 6-thioguanine and sensitive to HAT medium.
This line was derived from C32 (ATCC CRL-1585 ) by selection for 6-thioquanune resistance.
They are deficient in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-).
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, 90%; fetal bovine serum, 10%; 0.050 mM 6-thioguanine
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution several minutes until cells detach. Add fresh medium and centrifuge. Resuspend in fresh medium and dispense into new flasks.
Related Products:derivative:ATCC CRL-1585
References: 1082: Chen TR. Chromosome changes in 6-TG-resistant mutant strains derived from a karyotypically stable human line, C32. Cytogenet. Cell Genet. 35: 181-189, 1983. PubMed: 6861522
26091: Chen TR. Evolution in vitro of stemlines with minimal karyotypic deviations in a human heteroploid cell line. J. Natl. Cancer Inst. 61: 277-284, 1978. PubMed: 277716
26092: Chen TR, Shaw MW. Stable chromosome changes in human malignant melanoma. Cancer Res. 33: 2042-2047, 1973. PubMed: 4725368
