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平臺(tái)編號(hào)：bio-69434
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國(guó)際編號(hào)：CRL-1532?
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細(xì)胞信息： 182-PF SK
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
182-PF SK
ATCC Number：CRL-1532?
相關(guān)疾?。浩渌膊?br /> 運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
細(xì)胞形態(tài)：成纖維樣
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
年限：20 years
器官來(lái)源：皮膚
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
規(guī)格：50次 Designations: 182-PF SK
Depositors: ?L Kopelovich
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: hereditary adenomatosis
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 20 years
Gender: male
Ethnicity: Caucasian
Comments:The tissue donor suffered from hereditary adenomatosis.
The line is capable of 10 population doublings after thawing.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 days.
References: 22736: Rhim JS, et al. Chemical transformation of cultured skin fibroblasts from humans genetically predisposed to cancer. Cancer Detect. Prev. 4: 239-247, 1981. PubMed: 7349781
22759: . . Prog. Cancer Res. Ther. 13: 97-108, 1980.
相關(guān)疾?。浩渌膊?br /> 運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
細(xì)胞形態(tài)：成纖維樣
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
年限：20 years
器官來(lái)源：皮膚
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
規(guī)格：50次 Designations: 182-PF SK
Depositors: ?L Kopelovich
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: hereditary adenomatosis
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 20 years
Gender: male
Ethnicity: Caucasian
Comments:The tissue donor suffered from hereditary adenomatosis.
The line is capable of 10 population doublings after thawing.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 days.
References: 22736: Rhim JS, et al. Chemical transformation of cultured skin fibroblasts from humans genetically predisposed to cancer. Cancer Detect. Prev. 4: 239-247, 1981. PubMed: 7349781
22759: . . Prog. Cancer Res. Ther. 13: 97-108, 1980.
