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平臺編號：bio-69432
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國際編號：CRL-2394?
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細胞信息： GA-10 (Clone 20)
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規(guī)格：Frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
GA-10 (Clone 20)
生長狀態(tài)：懸浮生長
ATCC Number：CRL-2394?
相關(guān)疾?。毫馨土?br /> 數(shù)量：大量
運輸方式：凍存運輸
細胞形態(tài)：淋巴樣
年限：41 years
細胞類型：B淋巴細胞
是否是腫瘤細胞：1
物種來源：人
規(guī)格：120 ml Designations: GA-10 (Clone 20)
Depositors: ?AP Rapoport
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens deposited as human
Morphology:lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD77 + (80-90%)
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,20
Age: 41 years
Gender: male
Ethnicity: Caucasian, White
Comments:GA-10 (Clone 20) is a subclone of the GA-10 (ATCC CRL-2392 ) cell line derived by culturing the parental line on semi-solid medium conditioned by a feeder layer.
GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.
The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy).
This clone of cells also expresses high levels of CD77 (80-90%); CD77 is also known as Burkitt's lymphoma antigen (BLA).
GA-10 (Clone-20) cells exhibit marked sensitivity to Shiga Toxin (Stx) in cytotoxicity studies.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derived from same cell line:ATCC CRL-2392
derived from same cell line:ATCC CRL-2393
ATCC Number：CRL-2394?
相關(guān)疾?。毫馨土?br /> 數(shù)量：大量
運輸方式：凍存運輸
細胞形態(tài)：淋巴樣
年限：41 years
細胞類型：B淋巴細胞
是否是腫瘤細胞：1
物種來源：人
規(guī)格：120 ml Designations: GA-10 (Clone 20)
Depositors: ?AP Rapoport
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens deposited as human
Morphology:lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD77 + (80-90%)
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,20
Age: 41 years
Gender: male
Ethnicity: Caucasian, White
Comments:GA-10 (Clone 20) is a subclone of the GA-10 (ATCC CRL-2392 ) cell line derived by culturing the parental line on semi-solid medium conditioned by a feeder layer.
GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.
The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy).
This clone of cells also expresses high levels of CD77 (80-90%); CD77 is also known as Burkitt's lymphoma antigen (BLA).
GA-10 (Clone-20) cells exhibit marked sensitivity to Shiga Toxin (Stx) in cytotoxicity studies.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derived from same cell line:ATCC CRL-2392
derived from same cell line:ATCC CRL-2393
