運(yùn)輸方式：凍存運(yùn)輸
器官來源：腎臟
生長狀態(tài)：貼壁生長
ATCC Number：CRL-1440?
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細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：1
物種來源：人
年限：9 months
數(shù)量：大量
規(guī)格：10次 Designations: G-402
Depositors: ?PT Peebles
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial-like
Source: Organ: kidney
Disease: renal leiomyoblastoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis:This is a human cell line with the hyperdiploid chromosome count. The modal chromosome number was 47, occurring in 40% of cells. The rate of higher ploidies was 0.3%. The karyotype was 47,XX,+2,-5,+?ins(4) (q11.2) for the modal cell population. No other karyotypic abnormalities were evident.
Isoenzymes: G6PD, B
Age: 9 months
Gender: female
Ethnicity: Caucasian
Comments:The line will grow in soft agar.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
