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平臺編號：bio-69429
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國際編號：CRL-10423?
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細(xì)胞信息： JM1
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
JM1
細(xì)胞類型：其他細(xì)胞類型
數(shù)量：大量
是否是腫瘤細(xì)胞：1
物種來源：人
運輸方式：凍存運輸
ATCC Number：CRL-10423?
相關(guān)疾?。浩渌膊?br /> 生長狀態(tài)：懸浮生長
細(xì)胞形態(tài)：淋巴樣
規(guī)格：200次 Designations: JM1
Depositors: ?Immunex Corporation
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: B cell lymphoma
Cell Type: pre-B lymphoblast;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:HLA-DR; Homo sapiens, expressed
CD19; Homo sapiens, expressed
CD10; Homo sapiens, expressed
DNA Profile (STR):D5S818: 12
D13S317: 11
D7S820: 8, 10
D16S539: 9
vWA: 17
THO1: 6
TPOX: 10, 11
CSF1PO: 12
Amelogenin: X Y
Comments:The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia.The cells are negative for CD7, My 7, My 9 and surface IgM.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures may be established by centrifugation. Adherent cells can be dislodged by scraping. Initiate cultures at 2 to 5 x 10(5) viable cells/ml. Subculture when cell density reaches 1 X 10(6) cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 95%; DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
References: 91916: Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717
數(shù)量：大量
是否是腫瘤細(xì)胞：1
物種來源：人
運輸方式：凍存運輸
ATCC Number：CRL-10423?
相關(guān)疾?。浩渌膊?br /> 生長狀態(tài)：懸浮生長
細(xì)胞形態(tài)：淋巴樣
規(guī)格：200次 Designations: JM1
Depositors: ?Immunex Corporation
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: B cell lymphoma
Cell Type: pre-B lymphoblast;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:HLA-DR; Homo sapiens, expressed
CD19; Homo sapiens, expressed
CD10; Homo sapiens, expressed
DNA Profile (STR):D5S818: 12
D13S317: 11
D7S820: 8, 10
D16S539: 9
vWA: 17
THO1: 6
TPOX: 10, 11
CSF1PO: 12
Amelogenin: X Y
Comments:The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia.The cells are negative for CD7, My 7, My 9 and surface IgM.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
- 0.05 mM 2-mercaptoethanol
- fetal bovine serum to a final concentration of 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures may be established by centrifugation. Adherent cells can be dislodged by scraping. Initiate cultures at 2 to 5 x 10(5) viable cells/ml. Subculture when cell density reaches 1 X 10(6) cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 95%; DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
References: 91916: Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717
