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平臺編號：bio-69424
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國際編號：CRL-8304?
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細胞信息： 143B PML BK TK
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規(guī)格：Frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
143B PML BK TK
年限：13 year old
運輸方式：凍存運輸
器官來源：骨
ATCC Number：CRL-8304?
相關疾病：骨肉瘤
是否是腫瘤細胞：1
物種來源：人
細胞形態(tài)：成纖維樣
數(shù)量：大量
生長狀態(tài)：貼壁生長
規(guī)格：10 μg Designations: 143B PML BK TK
Depositors: ?Wistar Institute
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
Age: 13 year old
Gender: female
Ethnicity: Caucasian
Comments:Contains a thymidine kinase (TK+) plasmid (PMBKTK).
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products:plasmid in bacteria:ATCC 39369
parental cell line:ATCC CRL-8303
運輸方式：凍存運輸
器官來源：骨
ATCC Number：CRL-8304?
相關疾病：骨肉瘤
是否是腫瘤細胞：1
物種來源：人
細胞形態(tài)：成纖維樣
數(shù)量：大量
生長狀態(tài)：貼壁生長
規(guī)格：10 μg Designations: 143B PML BK TK
Depositors: ?Wistar Institute
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
Age: 13 year old
Gender: female
Ethnicity: Caucasian
Comments:Contains a thymidine kinase (TK+) plasmid (PMBKTK).
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products:plasmid in bacteria:ATCC 39369
parental cell line:ATCC CRL-8303
