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平臺編號：bio-69418
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國際編號：CRL-1424?
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細(xì)胞信息： G-361
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
G-361
數(shù)量：大量
器官來源：皮膚
是否是腫瘤細(xì)胞：1
物種來源：人
ATCC Number：CRL-1424?
相關(guān)疾?。簮盒院谏亓?br /> 細(xì)胞形態(tài)：上皮樣
年限：31 years adult
運輸方式：凍存運輸
規(guī)格：100 ul Designations: G-361
Depositors: ?PT Peebles
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Organism: Homo sapiens
Morphology:epithelial
Source: Organ: skin
Disease: malignant melanoma
Cellular Products:melanin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 15
D5S818: 11
D7S820: 8,11
THO1: 7,9
TPOX: 8,10
vWA: 15,17
Isoenzymes: G6PD, B
Age: 31 years adult
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 24 hours after seeding from a thawed ampule, then 2 to 3 times per week
References: 22209: . . Pediatr. Res. 12: 485, 1978.
器官來源：皮膚
是否是腫瘤細(xì)胞：1
物種來源：人
ATCC Number：CRL-1424?
相關(guān)疾?。簮盒院谏亓?br /> 細(xì)胞形態(tài)：上皮樣
年限：31 years adult
運輸方式：凍存運輸
規(guī)格：100 ul Designations: G-361
Depositors: ?PT Peebles
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Organism: Homo sapiens
Morphology:epithelial
Source: Organ: skin
Disease: malignant melanoma
Cellular Products:melanin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 15
D5S818: 11
D7S820: 8,11
THO1: 7,9
TPOX: 8,10
vWA: 15,17
Isoenzymes: G6PD, B
Age: 31 years adult
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 24 hours after seeding from a thawed ampule, then 2 to 3 times per week
References: 22209: . . Pediatr. Res. 12: 485, 1978.
