-
平臺編號：bio-69413
-
國際編號：TIB-223?
-
細胞信息： GCT
-
規(guī)格：Frozen
-
用途：ATCC原裝細胞
-
服務(wù)費用：
加載中…… - 訂購
-
注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
GCT
運輸方式：凍存運輸
年限：29 years
ATCC Number：TIB-223?
相關(guān)疾?。浩渌膊?br /> 生長狀態(tài)：貼壁生長
數(shù)量：大量
是否是腫瘤細胞：1
物種來源：人
規(guī)格：500 ml Designations: GCT [Giant Cell Tumor]
Depositors: ?J Brennan
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:
Source: Disease: fibrous histiocytoma
Derived from metastatic site: lung
Cellular Products:colony stimulating activity (CSA); erythroid enhancing activity (EEA); prostaglandin E; plasminogen activator
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 9
D5S818: 13,15
D7S820: 11,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,18
Age: 29 years
Gender: male
Comments:The line produces CSA for human granulocyte precursors and EEA for erythroid precursor.
Medium conditioned by this line can be used as a source of prostaglandin E and plasminogen activator.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin for 2 to 3 minutes, add fresh medium, aspirate and dispense into new flasks.
References: 1181: Di Persio JF, et al. Human cell lines that elaborate colony-stimulating activity for the marrow cells of man and other species. Blood 51: 507-519, 1978. PubMed: 304748
1182: DiPersio JF, et al. The fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT. Blood 56: 717-727, 1980. PubMed: 6968234
1183: Abboud CN, et al. Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT. Blood 58: 1148-1154, 1981. PubMed: 6975643
3550: Lichtman MA, Brennan JK. Obtaining human cell lines that elaborate colony stimulating activity for marrow cells of man and other species and methods of preparing same. US Patent 4,135,975 dated Jan 23 1979
58064: Abboud CN, et al. Erythropoietic enhancing activity (EEA) secreted by the human cell line, GCT. J. Supramol. Struct. 13: 199-209, 1980. PubMed: 6972469
年限：29 years
ATCC Number：TIB-223?
相關(guān)疾?。浩渌膊?br /> 生長狀態(tài)：貼壁生長
數(shù)量：大量
是否是腫瘤細胞：1
物種來源：人
規(guī)格：500 ml Designations: GCT [Giant Cell Tumor]
Depositors: ?J Brennan
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:
Source: Disease: fibrous histiocytoma
Derived from metastatic site: lung
Cellular Products:colony stimulating activity (CSA); erythroid enhancing activity (EEA); prostaglandin E; plasminogen activator
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 9
D5S818: 13,15
D7S820: 11,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,18
Age: 29 years
Gender: male
Comments:The line produces CSA for human granulocyte precursors and EEA for erythroid precursor.
Medium conditioned by this line can be used as a source of prostaglandin E and plasminogen activator.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin for 2 to 3 minutes, add fresh medium, aspirate and dispense into new flasks.
References: 1181: Di Persio JF, et al. Human cell lines that elaborate colony-stimulating activity for the marrow cells of man and other species. Blood 51: 507-519, 1978. PubMed: 304748
1182: DiPersio JF, et al. The fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT. Blood 56: 717-727, 1980. PubMed: 6968234
1183: Abboud CN, et al. Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT. Blood 58: 1148-1154, 1981. PubMed: 6975643
3550: Lichtman MA, Brennan JK. Obtaining human cell lines that elaborate colony stimulating activity for marrow cells of man and other species and methods of preparing same. US Patent 4,135,975 dated Jan 23 1979
58064: Abboud CN, et al. Erythropoietic enhancing activity (EEA) secreted by the human cell line, GCT. J. Supramol. Struct. 13: 199-209, 1980. PubMed: 6972469
