是否是腫瘤細胞：1
物種來源：人
運輸方式：凍存運輸
ATCC Number：CCL-224?
相關疾?。捍竽c癌
器官來源：結腸
生長狀態(tài)：懸浮生長，少量貼壁
細胞形態(tài)：其他
年限：Dukes' type D
數(shù)量：大量
規(guī)格：48T Designations: COLO 201
Depositors: ?GE Moore
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, with some loosely adherent cells
Organism: Homo sapiens
Morphology:bipolar, slightly refractile, fibroblast-like cell
Source: Organ: colon
Tumor Stage: Dukes' type D
Disease: colorectal adenocarcinoma
Derived from metastatic site: ascites
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Oncogene:myc +; ras + ; myb + ; fos +; sis +; p53 +; abl -; ros -; src -
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 10,12
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
THO1: 8,9
TPOX: 11
vWA: 15
Cytogenetic Analysis:The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic.
Isoenzymes: ES-D, 1-2
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1-2
PGM3, 1-2
Age: 70 years
Gender: male
Ethnicity: Caucasian
Comments:The line is negative for CSAp (CSAp-) and CEA.
It was derived from tissue from the same patient as COLO 205 (ATCC CCL-222 ).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a 0.25% trypsin, 0.53 mM EDTA solution. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derived from same individual:ATCC CCL-222
derived from same individual:ATCC CCL-224
References: 1046: Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251
22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
