ATCC Number：HTB-178?
相關(guān)疾?。浩渌膊?br />
細(xì)胞形態(tài)：上皮樣
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
器官來(lái)源：肺
運(yùn)輸方式：凍存運(yùn)輸
年限：73 years
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
數(shù)量：大量
規(guī)格：0.1ml Designations: NCI-H596 [H596]
Depositors: ?AF Gazdar, JD Minna
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: lung
Disease: adenosquamous carcinoma
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Isolation: Isolation date: 1983
Applications:transfection host (Roche Transfection Reagents )
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 12,13
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 11
THO1: 7,9.3
TPOX: 8
vWA: 16
Cytogenetic Analysis:modal number = 71; range = 65 to 75.
This is a near triploid human cell line. The modal chromosome number was 71 although cells with 70 chromosomes occurred frequently. The frequency of higher ploidies was 2.9%.; Over 11 marker chromosomes were common to all cells, and at least 6 others were found in some cells. Among the common markers were: del(1)(p22), i(3q), i(6p), i(7p), t(7p17q), 14q+ and five others.; Of these, del(1) and i(7p) were generally double [occasionally triple for del(1)]. Constantly, there were 2 to 3 minute chromosomes that were about 1/2 the size of a G group chromosome.; Structurally normal N17 had only a single copy per cell, whereas N15 had four copies and N20 and N21 had four or more copies. The X chromosome was paired, but the Y was not found by fluorescence microscopy.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 1
Age: 73 years
Gender: male
Ethnicity: Caucasian
Comments:The NCI-H596 cell line was derived by A.F. Gazdar and H. Oie in 1983 from a tumor mass in the chest wall of a patient with adenosquamous carcinoma of the lung.
The specimen was obtained prior to therapy.
The cells express easily detectable levels of p53 mRNA (comparable to levels found in normal lung), and exhibit no gross structural DNA abnormalities.
The cells stain positively for keratin and vimentin, but are negative for neurofilament triplet protein.
The cells express multiple markers of squamous differentiation, including cross linked envelope formation, transglutaminase activity and production of involucrin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876
1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
22437: Levitt ML, et al. Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990. PubMed: 1967140
