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平臺編號：bio-69364
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國際編號：HTB-121?
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細胞信息： BT-483
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規(guī)格：Frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
BT-483
年限：23 years
細胞形態(tài)：上皮樣
器官來源：乳房
運輸方式：凍存運輸
生長狀態(tài)：貼壁生長
ATCC Number：HTB-121?
相關(guān)疾?。簩?dǎo)管癌
數(shù)量：大量
是否是腫瘤細胞：1
物種來源：人
規(guī)格：60 次 Designations: BT-483
Depositors: ?EY Lasfargues
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: mammary gland; breast
Disease: ductal carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 11,12
D5S818: 11
D7S820: 9,10
THO1: 5,9.3
TPOX: 8,11
vWA: 17
Cytogenetic Analysis:modal number = 72; range = 46 to 130
Isoenzymes: AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 2
Me-2, 0
PGM1, 2
PGM3, 2
Age: 23 years
Gender: female
Ethnicity: Caucasian
Comments:The cells were highly pleomorphic with prominent nuclei and nucleoli [PubMed ID: 212572]. Epithelial characteristics including desmosomes, microvilli, tight and gap junctions were observed by transmission electron microscopy [PubMed ID: 212572].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
purified DNA:ATCC HTB-121D
References: 22542: Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572
23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
細胞形態(tài)：上皮樣
器官來源：乳房
運輸方式：凍存運輸
生長狀態(tài)：貼壁生長
ATCC Number：HTB-121?
相關(guān)疾?。簩?dǎo)管癌
數(shù)量：大量
是否是腫瘤細胞：1
物種來源：人
規(guī)格：60 次 Designations: BT-483
Depositors: ?EY Lasfargues
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: mammary gland; breast
Disease: ductal carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 11,12
D5S818: 11
D7S820: 9,10
THO1: 5,9.3
TPOX: 8,11
vWA: 17
Cytogenetic Analysis:modal number = 72; range = 46 to 130
Isoenzymes: AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 2
Me-2, 0
PGM1, 2
PGM3, 2
Age: 23 years
Gender: female
Ethnicity: Caucasian
Comments:The cells were highly pleomorphic with prominent nuclei and nucleoli [PubMed ID: 212572]. Epithelial characteristics including desmosomes, microvilli, tight and gap junctions were observed by transmission electron microscopy [PubMed ID: 212572].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
purified DNA:ATCC HTB-121D
References: 22542: Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572
23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
