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ATCC Number：CRL-1675?
相關(guān)疾病：黑色素瘤
年限：58 years adult
是否是腫瘤細胞：1
物種來源：人
運輸方式：凍存運輸
器官來源：皮膚
細胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
數(shù)量：大量
規(guī)格：10 μg Designations: WM-115
Depositors: ?M Herlyn
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: skin
Disease: melanoma
Cellular Products:proteoglycan antigen characteristic of melanoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,12
D5S818: 13
D7S820: 8
THO1: 7,9
TPOX: 8,11
vWA: 15,17
Cytogenetic Analysis:This is a hypotetraploid human cell line. The modal chromosome number is 87, occurring at 50%, with higher ploidies at 1.6%. Eight markers [der(1)t(1;9) (p31.2;q11), 6p+, del(6) (p21.1), t(12p14q) and four others] were found in all cells. Of these markers, six (including all four listed above) were consistently paired and two others were single. Normal N6 and N9 were absent and N7 had six copies per cell. There were only two X chromosomes per cell.
Age: 58 years adult
Gender: female
Comments:The WM-115 cell line was derived from the primary tumor.
Another cell line derived from a metastasis in the same patient also is available (ATCC CRL-1676 ).
The cells should be incubated at 34C to 35C.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 34.0°C; (Max. 35.0 °C, Min. 34.0°C)
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.03% EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 34 to 35?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 34 to 35?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
derived from same individual:ATCC CRL-1676