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平臺編號：bio-69311
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國際編號：CRL-1473?
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細(xì)胞信息： HT-1197
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
HT-1197
年限：44 years
生長狀態(tài)：貼壁生長
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-1473?
相關(guān)疾?。耗[瘤
器官來源：膀胱
數(shù)量：大量
是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：60 次×3 Designations: HT-1197
Depositors: ?S Rasheed
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:
Source: Organ: urinary bladder
Disease: carcinoma
Cellular Products:fibrinolytic activity
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Isoenzymes: G6PD, B
Age: 44 years
Gender: male
Ethnicity: Caucasian
Comments:The cells will also grow in soft agar.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37?C.
Subcultivation Ratio: Subcultivation Ratio: 1:5 to 1:10
Medium Renewal: Twice per week
Preservation: Storage temperature: liquid nitrogen vapor phase
Related Products:purified DNA:ATCC CRL-1473D
References: 22538: Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628
生長狀態(tài)：貼壁生長
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-1473?
相關(guān)疾?。耗[瘤
器官來源：膀胱
數(shù)量：大量
是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：60 次×3 Designations: HT-1197
Depositors: ?S Rasheed
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:
Source: Organ: urinary bladder
Disease: carcinoma
Cellular Products:fibrinolytic activity
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Isoenzymes: G6PD, B
Age: 44 years
Gender: male
Ethnicity: Caucasian
Comments:The cells will also grow in soft agar.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37?C.
Subcultivation Ratio: Subcultivation Ratio: 1:5 to 1:10
Medium Renewal: Twice per week
Preservation: Storage temperature: liquid nitrogen vapor phase
Related Products:purified DNA:ATCC CRL-1473D
References: 22538: Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628
