數(shù)量：大量
ATCC Number：CRL-1647?
相關(guān)疾?。毫馨土?br />
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：1
物種來源：人
生長狀態(tài)：懸浮生長
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
組織來源：ascites
規(guī)格：100 ul Designations: ST486
Depositors: ?IT Magrath
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Tissue: ascites
Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Cellular Products:IgM; kappa light chain
immunoglobulin (surface and secreted)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:complement
Antigen Expression:HLA A2, A10 (Aw25), B5 (Bw52), B18
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 8,12
D16S539: 9,12
D5S818: 11,12
D7S820: 7,12
THO1: 7,9
TPOX: 8,11
vWA: 17,18
Gender: female
Comments:The cells are EBNA negative and are negative for Fc receptors.
Approximately 2% of the cells express commplement receptors.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 19 to 20 hrs
References: 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763
22548: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721
22549: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722
