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年限：26 years
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：1
物種來源：人
ATCC Number：TIB-162?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。浩渌膊?br /> 生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)，多細(xì)胞聚集 Designations: HuT 102
Depositors: ?AF Gazdar
Biosafety Level:2 [Cells Contain RETROVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, multicell aggregates
Organism: Homo sapiens
Morphology:lymphoblast


Source: Disease: lymphoma; mycosis fungoides
Cell Type: cutaneous T lymphocyte;
Cellular Products:HTLV-I (human T cell leukemia virus)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:interleukin 2 (IL-2)
Antigen Expression:CD4; Homo sapiens
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 8,11
D13S317: 11,13
D16S539: 12
D5S818: 8,13
D7S820: 8,10
THO1: 7,8
TPOX: 6
vWA: 16,19
Age: 26 years
Gender: male
Ethnicity: Black
Comments:The Hut 102 cell line was derived from the peripheral blood of a 26 year old Black male patient with mycosis fungoides.
This line has the properties of a mature human T cell with helper/inducer activity.
It has receptors for IL-2, and the growth rate is stimulated by IL-2.
The line is E-rosette positive (E+), and negative for Epstein-Barr virus nuclear antigen (EBNA-), surface immunoglobulin (sIg-) and terminal deoxynucleotidyl transferase (TdT-).
The cells release a type C retrovirus that has been associated with human T cell lymphomas and should be handled as a biohazard.
The line was originally thought to produce IL-2; however, recent studies have failed to detect IL-2 mRNA production in Hut 102 cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.Maintain cell density between 2 X 10 exp5 and 8 X 10 exp5 viable cells/ml. Do not allow density to exceed 1 X 10 exp6 cells/ml. Background debris is often present.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Doubling Time: about 41 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1140: Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346
22610: Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013
23184: Hsu SM, et al. Expression of p55 (Tac) interleukin-2 receptor (IL-2R), but not p75 IL- 2R, in cultured H-RS cells and H-RS cells in tissues. Am. J. Pathol. 136: 735-744, 1990. PubMed: 1691591
23271: Poiesz BJ, et al. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc. Natl. Acad. Sci. USA 77: 7415-7419, 1980. PubMed: 6261256
23311: Wano Y, et al. Stable expression of the tax gene of type I human T-cell leukemia virus in human T cells activates specific cellular genes involved in growth. Proc. Natl. Acad. Sci. USA 85: 9733-9737, 1988. PubMed: 3059351