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平臺編號：bio-69221
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國際編號：HTB-15?
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細(xì)胞信息： U-118 MG
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用及說明書：
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
U-118 MG
數(shù)量：大量
器官來源：大腦
運(yùn)輸方式：凍存運(yùn)輸
生長狀態(tài)：貼壁生長
年限：classified as grade IV as of 2007
細(xì)胞形態(tài)：混合型
ATCC Number：HTB-15?
相關(guān)疾?。浩渌膊?br /> 是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：100次 Designations: U-118 MG
Depositors: ?J Ponten
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:mixed
Source: Organ: brain
Tumor Stage: classified as grade IV as of 2007
Disease: glioblastoma; astrocytoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:Blood Type A, Rh+; HLA Aw24, A28, B12, Bw47
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18
Isoenzymes: AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 2
PGM3, 2
Age: 50 years
Gender: male
Ethnicity: Caucasian
Comments:NOTE: The two glioblastoma cell lines, U-118 MG (HTB-15) and U-138 MG (HTB-16), reportedly from different individuals have identical VNTR and similar STR patterns.
U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes.
This is one of a number of cell lines derived from malignant gliomas (see also ATCC HTB-14 and ATCC HTB-16 ) by J. Ponten and associates from 1966 to 1969.
Mycoplasma contamination was eliminated in 1987 by treatment with BM-Cycline over a six week culture period.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapot temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22159: Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
23128: Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504
23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
23260: Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 75: 3965-3969, 1978. PubMed: 279013
32276: Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760
器官來源：大腦
運(yùn)輸方式：凍存運(yùn)輸
生長狀態(tài)：貼壁生長
年限：classified as grade IV as of 2007
細(xì)胞形態(tài)：混合型
ATCC Number：HTB-15?
相關(guān)疾?。浩渌膊?br /> 是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：100次 Designations: U-118 MG
Depositors: ?J Ponten
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:mixed
Source: Organ: brain
Tumor Stage: classified as grade IV as of 2007
Disease: glioblastoma; astrocytoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:Blood Type A, Rh+; HLA Aw24, A28, B12, Bw47
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18
Isoenzymes: AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 2
PGM3, 2
Age: 50 years
Gender: male
Ethnicity: Caucasian
Comments:NOTE: The two glioblastoma cell lines, U-118 MG (HTB-15) and U-138 MG (HTB-16), reportedly from different individuals have identical VNTR and similar STR patterns.
U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes.
This is one of a number of cell lines derived from malignant gliomas (see also ATCC HTB-14 and ATCC HTB-16 ) by J. Ponten and associates from 1966 to 1969.
Mycoplasma contamination was eliminated in 1987 by treatment with BM-Cycline over a six week culture period.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapot temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22159: Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23094: Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
23128: Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504
23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
23260: Bluestein HG. Neurocytotoxic antibodies in serum of patients with systemic lupus erythematosus. Proc. Natl. Acad. Sci. USA 75: 3965-3969, 1978. PubMed: 279013
32276: Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760
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