年限：6 years juvenile
細胞形態(tài)：上皮樣
數(shù)量：大量
是否是腫瘤細胞：1
物種來源：人
器官來源：大腦
生長狀態(tài)：懸浮生長
運輸方式：凍存運輸
ATCC Number：HTB-185?
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組織來源：cerebellum
規(guī)格：60 次×5 Designations: D283 Med
Depositors: ?HS Friedman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, multicell aggregates and some adherent cells
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: brain
Tissue: cerebellum
Disease: medulloblastoma
Derived from metastatic site: peritoneum
Cellular Products:neurofilament proteins (NFP)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1985
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 9,12
D13S317: 8,10
D16S539: 11
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 8,11
vWA: 16,18
Cytogenetic Analysis:The karyotype is 45, XY, -7, -8, -17, -20, der(20)t(1;20)(q12;q13), 8q+, 17p+ (range = 41 to 46). This is a hypodiploid cell line with a frequency of higher ploidies of 5.4%. Three marker chromosomes are present in all cells. They are: der(20)t(1;20)(q12;q13), 8q+ and 17p+. N7, N17 and N20 have single copies. The single X is structurally normal, and the Y chromosome is present as confirmed by fluorescence microscopy.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 0
PGM1, 1
PGM3, 1
Age: 6 years juvenile
Gender: male
Ethnicity: Caucasian
Comments:The D283 Med cell line was established in 1985 by Friedman et al. from malignant ascites cells and a peritoneal metastasis from a boy with medulloblastoma. The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 10(5) viable cells/ml. Maintain cultures between 4 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 52 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 2096: Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793
23092: He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373
23157: Friedman HS, et al. Establishment and characterization of the human medulloblastoma cell line and transplantable xenograft D283 Med. J. Neuropathol. Exp. Neurol. 44: 592-605, 1985. PubMed: 4056828
23181: Trojanowski JQ, et al. A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits. Am. J. Pathol. 126: 358-363, 1987. PubMed: 3103453
32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024
