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平臺編號：bio-69187
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國際編號：HTB-13?
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細胞信息： SW 1783
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規(guī)格：Frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
SW 1783
器官來源：大腦
年限：grade III
是否是腫瘤細胞：1
物種來源：人
生長狀態(tài)：貼壁生長
ATCC Number：HTB-13?
相關(guān)疾病：其他疾病
運輸方式：凍存運輸
數(shù)量：大量
細胞形態(tài)：成纖維樣
規(guī)格：100 ul Designations: SW 1783 [SW-1783, SW1783]
Depositors: ?A Leibovitz
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: brain
Tumor Stage: grade III
Disease: astrocytoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Temple Texas, United States
Isolation date: 1977
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,13
D13S317: 9,11
D16S539: 12
D5S818: 12,13
D7S820: 9,13
THO1: 6,8
TPOX: 8,11
vWA: 16,17
Cytogenetic Analysis:hypertetraploid; modal number = 96. The rate of higher ploidies was 10.1%. The markers der(5)t(1;5) (p11;p15), t(1q3q), del(3) (q21), der(6)t(6;11) (p12;p15), der(11)t(6;11) (p12;p15), and 5 others were common to most cells. Of these, der (6) and der(11) had consistently two copies per cell, and were probably formed by balanced translocation between N6 and N11. Nine to 10 marker chromosomes were common to some cells, and 10 to 15 others were detected only once. All normal chromosomes were present in 2 to 5 copies per cell. Generally X had 3 copies and Y had 2 copies per cell. Double minutes (DM) were seen in some cells, and only 1 to a few copies occurred per cell.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 1
Age: 68 years adult
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
References: 32274: Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765
年限：grade III
是否是腫瘤細胞：1
物種來源：人
生長狀態(tài)：貼壁生長
ATCC Number：HTB-13?
相關(guān)疾病：其他疾病
運輸方式：凍存運輸
數(shù)量：大量
細胞形態(tài)：成纖維樣
規(guī)格：100 ul Designations: SW 1783 [SW-1783, SW1783]
Depositors: ?A Leibovitz
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: brain
Tumor Stage: grade III
Disease: astrocytoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Temple Texas, United States
Isolation date: 1977
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,13
D13S317: 9,11
D16S539: 12
D5S818: 12,13
D7S820: 9,13
THO1: 6,8
TPOX: 8,11
vWA: 16,17
Cytogenetic Analysis:hypertetraploid; modal number = 96. The rate of higher ploidies was 10.1%. The markers der(5)t(1;5) (p11;p15), t(1q3q), del(3) (q21), der(6)t(6;11) (p12;p15), der(11)t(6;11) (p12;p15), and 5 others were common to most cells. Of these, der (6) and der(11) had consistently two copies per cell, and were probably formed by balanced translocation between N6 and N11. Nine to 10 marker chromosomes were common to some cells, and 10 to 15 others were detected only once. All normal chromosomes were present in 2 to 5 copies per cell. Generally X had 3 copies and Y had 2 copies per cell. Double minutes (DM) were seen in some cells, and only 1 to a few copies occurred per cell.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 1
Age: 68 years adult
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
References: 32274: Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765
