細胞形態(tài)：上皮樣
數(shù)量：大量
器官來源：子宮
是否是腫瘤細胞：1
物種來源：人
年限：71 years
組織來源：endometrium
ATCC Number：HTB-113?
相關疾?。合侔?br />
生長狀態(tài)：貼壁生長
運輸方式：凍存運輸
規(guī)格：0.1mg Designations: HEC-1-B
Depositors: ?H Kuramoto
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: uterus
Tissue: endometrium
Disease: adenocarcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host (Roche Transfection Reagents )
Tumorigenic:Yes
Antigen Expression:Blood Type B; Rh+
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 11,16
D16S539: 11,12
D5S818: 11,13
D7S820: 9,11
THO1: 6,7
TPOX: 8,11
vWA: 18
Cytogenetic Analysis:diploid to tetraploid with large submetacentric marker
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1-2
Age: 71 years
Gender: female
Comments:This is a substrain of HEC-1-A (see ATCC HTB-112 ) isolated in 1968 by H. Kuramoto.
Unlike HEC-1-A, this substrain exhibited a stationary growth period between the 135th and 190th days in culture and appeared on recovery to be flattened and more pavement patterned than the parent line.
Futhermore, the predominant complement of chromosomes was double that observed for the parent line.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22449: Kuramoto H. Studies of the growth and cytogenetic properties of human endometrial adenocarcinoma in culture and its development into an established line. Acta Obstet. Gynaecol. Jpn. 19: 47-58, 1972. PubMed: 4678779
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23069: Presta M, et al. Modulation of plasminogen activator activity in human endometrial adenocarcinoma cells by basic fibroblast growth factor and transforming growth factor beta. Cancer Res. 48: 6384-6389, 1988. PubMed: 3263185
23541: Kuramoto H, et al. Establishment of a cell line of human endometrial adenocarcinoma in vitro. Am. J. Obstet. Gynecol. 114: 1012-1019, 1972. PubMed: 4673779
32299: St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830
33112: Schramm N, et al. Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect. Immun. 64: 1208-1214, 1996. PubMed: 8606080
