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年限：grade IV
細胞形態(tài)：上皮樣
是否是腫瘤細胞：1
物種來源：人
ATCC Number：HTB-5?
相關(guān)疾?。阂菩屑毎?br /> 數(shù)量：大量
運輸方式：凍存運輸
生長狀態(tài)：貼壁生長
器官來源：膀胱
規(guī)格：15 ml Designations: TCCSUP
Depositors: ?C O'Toole
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: urinary bladder
Tumor Stage: grade IV
Disease: transitional cell carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:HLA 2, 3, 7, 12
DNA Profile (STR):Amelogenin: X
CSF1PO: 10
D13S317: 11,14
D16S539: 9,11
D5S818: 12
D7S820: 8,9
THO1: 6,9.3
TPOX: 8
vWA: 14,16
Cytogenetic Analysis:(P12 and 35) hypotetraploid with marker chromosomes
Isoenzymes: AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 2
PGM3, 1
Age: 67 years
Gender: female
Comments:The TCCSUP line was isolated in 1974 from an anaplastic transitional cell carcinoma (TCC) in the neck of the urinary bladder.
The patient had a 4 month history of hematuria prior to removal of the tumor.
Metastases to the bone marrow were discovered later.
Studies on ultrastructure indicated presence of microvilli and lipid bodies but no desmosomes were observed.
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
4. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37C. Subculture every 6 to 8 days.

Preservation: Culture medium, 95%; DMSO, 5%
Related Products:purified DNA:ATCC HTB-5D
References: 21849: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.
24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484
26317: Nayak SK, et al. A cell line from an anaplastic transitional cell carcinoma of human urinary bladder. Br. J. Cancer 35: 142-151, 1977. PubMed: 836756