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平臺編號：bio-69153
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國際編號：HTB-183?
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細(xì)胞信息： NCI-H661
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
NCI-H661
ATCC Number：HTB-183?
相關(guān)疾?。浩渌膊?br /> 運(yùn)輸方式：凍存運(yùn)輸
年限：43 years
細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：1
物種來源：人
數(shù)量：大量
生長狀態(tài)：貼壁生長
器官來源：肺
規(guī)格：0.1ml Designations: NCI-H661 [H661]
Depositors: ?AF Gazdar, JD Minna
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: lymph node
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Bethesda Maryland,
Isolation date: 1982
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 17
Cytogenetic Analysis:hyperhexaploid; modal number = 142; range = 130 to 153. Each cell had over 70 marker chromosomes. Among the markers were t(1p10q), del(1)(q11/q12), 6q+, 2q+,der(20)t(12;20)(q11;q13). Some of these had 2 to copies per cell. Most cells also had 5 to 10 microchromosomes (metacentric like). Structurally normal N2,N4 and N9 were absent; two copies of the X and multiple copies of the Y were detected.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 0
PGM1, 1
PGM3, 1
Age: 43 years
Gender: male
Ethnicity: Caucasian
Comments:The line lacks ultrastructural and biochemical evidence of squamous differentiation or mucin production.
The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities.
The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA, rinse quickly and remove trypsin leaving 1 to 2 ml. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, disperse cells and transfer to a new flask.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876
1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
22437: Levitt ML, et al. Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990. PubMed: 1967140
相關(guān)疾?。浩渌膊?br /> 運(yùn)輸方式：凍存運(yùn)輸
年限：43 years
細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：1
物種來源：人
數(shù)量：大量
生長狀態(tài)：貼壁生長
器官來源：肺
規(guī)格：0.1ml Designations: NCI-H661 [H661]
Depositors: ?AF Gazdar, JD Minna
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: lung
Disease: carcinoma; large cell lung cancer
Derived from metastatic site: lymph node
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Bethesda Maryland,
Isolation date: 1982
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 17
Cytogenetic Analysis:hyperhexaploid; modal number = 142; range = 130 to 153. Each cell had over 70 marker chromosomes. Among the markers were t(1p10q), del(1)(q11/q12), 6q+, 2q+,der(20)t(12;20)(q11;q13). Some of these had 2 to copies per cell. Most cells also had 5 to 10 microchromosomes (metacentric like). Structurally normal N2,N4 and N9 were absent; two copies of the X and multiple copies of the Y were detected.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 0
PGM1, 1
PGM3, 1
Age: 43 years
Gender: male
Ethnicity: Caucasian
Comments:The line lacks ultrastructural and biochemical evidence of squamous differentiation or mucin production.
The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities.
The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA, rinse quickly and remove trypsin leaving 1 to 2 ml. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, disperse cells and transfer to a new flask.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876
1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
22437: Levitt ML, et al. Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990. PubMed: 1967140
