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平臺(tái)編號(hào)：bio-69133
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國(guó)際編號(hào)：CCL-234?
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細(xì)胞信息： SW1463
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
SW1463
年限：Dukes' type C
ATCC Number：CCL-234?
相關(guān)疾病：大腸癌
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
器官來(lái)源：直腸
細(xì)胞形態(tài)：上皮樣
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
規(guī)格：300 ul Designations: SW1463 [SW 1463, SW-1463]
Depositors: ?A Leibovitz
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: rectum
Tumor Stage: Dukes' type C
Disease: colorectal adenocarcinoma
Cellular Products:carcinoembryonic antigen (CEA) 223 ng/10 exp6 cells/10 days; keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:blood type A; Rh +
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11
D5S818: 13,14
D7S820: 9
THO1: 6,7
TPOX: 8,11
vWA: 16
Isoenzymes: ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1-2
Age: 66 years
Gender: female
Ethnicity: Caucasian
Comments:The line was derived from a grade II mucous producing and grade III solid tumor.
CSAp negative (CSAp-).
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37C. Add fresh medium, disperse cells and centrifuge to pellet the cells.
Resuspend in fresh medium and dispense into new flasks.
Subculture just prior to confluency (about 10 to 12 days).
References: 23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
ATCC Number：CCL-234?
相關(guān)疾病：大腸癌
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
器官來(lái)源：直腸
細(xì)胞形態(tài)：上皮樣
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
規(guī)格：300 ul Designations: SW1463 [SW 1463, SW-1463]
Depositors: ?A Leibovitz
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: rectum
Tumor Stage: Dukes' type C
Disease: colorectal adenocarcinoma
Cellular Products:carcinoembryonic antigen (CEA) 223 ng/10 exp6 cells/10 days; keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:blood type A; Rh +
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11
D5S818: 13,14
D7S820: 9
THO1: 6,7
TPOX: 8,11
vWA: 16
Isoenzymes: ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1-2
Age: 66 years
Gender: female
Ethnicity: Caucasian
Comments:The line was derived from a grade II mucous producing and grade III solid tumor.
CSAp negative (CSAp-).
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37C. Add fresh medium, disperse cells and centrifuge to pellet the cells.
Resuspend in fresh medium and dispense into new flasks.
Subculture just prior to confluency (about 10 to 12 days).
References: 23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
