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細胞形態(tài)：淋巴樣
品系：B10.BR
ATCC Number：CRL-1778?
相關(guān)疾?。毫馨土?br /> 運輸方式：凍存運輸
細胞類型：其他細胞類型
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
生長狀態(tài)：懸浮生長
規(guī)格：0.5mg Designations: LBRM TG6
Depositors: ?JW Larrick
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast


Source: Disease: lymphoma
Strain: B10.BR
Cell Type: T lymphocyte; radiation induced
Cellular Products:interleukin 2; IL-2; interleukin-2
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:the depositor has provided these cells for research purposes only and with the following restrictions: (1) no distribution should be made to third parties, (2) any proposed commercial use must be negotiated with the Cetus Corporation, 1400 Fifty-Third St., Emeryville, California 94608, (3) all papers reporting any use of these or derived clones should make direct reference to the original publication (J. Immunol. Methods 79:39-45, 1985)
Receptors:interleukin 1 (IL-1)
Comments:The LBRM TG6 line was derived from the LBRM 33 T cell lymphoma (see ATCC TIB-155 ).
The cells produce IL-2 in response to IL-1 and PHA.
These cells can be used to measure IL-1 activity in a very rapid and sensitive assay.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 0.02 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22341: Larrick JW, et al. An improved assay for the detection of interleukin 1. J. Immunol. Methods 79: 39-45, 1985. PubMed: 3873500