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平臺(tái)編號(hào)：bio-69066
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國(guó)際編號(hào)：TIB-155?
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細(xì)胞信息： LBRM-33 clone 4A2
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
LBRM-33 clone 4A2
品系：B10.BR
生長(zhǎng)狀態(tài)：懸浮，少量貼壁
細(xì)胞形態(tài)：淋巴樣
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：TIB-155?
相關(guān)疾?。毫馨土?br /> 規(guī)格：0.5mg Designations: LBRM-33 clone 4A2
Depositors: ?P Ralph
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension; some adherent cells
Organism: Mus musculus
Morphology:lymphoblast
Source: Strain: B10.BR
Disease: lymphoma
Cell Type: T lymphocyte; radiation induced
Cellular Products:interleukin-2 (interleukin 2, IL-2)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:This line was derived from a radiation induced T cell lymphoma.
Production of IL-2 is stimulated by T cell mitogens.
Peak levels are found in the culture medium after 24 hours of stimulation with either 1% PHA or 20 microgm/ml Con A.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml Adherent cells can be dislodged by scraping with subsequent resuspension as above.
Preservation: 90% FBS + 10% DMSO
References: 1139: Gillis S, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. III. The isolation and phenotypic characterization of interleukin-2 producing T cell lymphomas. J. Immunol. 125: 2570-2578, 1980. PubMed: 6968790
23535: Mochizuki D, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. IV. Purification of Interleukin 2 from a murine T cell lymphoma. J. Immunol. 125: 2579-2583, 1980. PubMed: 7000903
生長(zhǎng)狀態(tài)：懸浮，少量貼壁
細(xì)胞形態(tài)：淋巴樣
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：TIB-155?
相關(guān)疾?。毫馨土?br /> 規(guī)格：0.5mg Designations: LBRM-33 clone 4A2
Depositors: ?P Ralph
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension; some adherent cells
Organism: Mus musculus
Morphology:lymphoblast
Source: Strain: B10.BR
Disease: lymphoma
Cell Type: T lymphocyte; radiation induced
Cellular Products:interleukin-2 (interleukin 2, IL-2)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:This line was derived from a radiation induced T cell lymphoma.
Production of IL-2 is stimulated by T cell mitogens.
Peak levels are found in the culture medium after 24 hours of stimulation with either 1% PHA or 20 microgm/ml Con A.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml Adherent cells can be dislodged by scraping with subsequent resuspension as above.
Preservation: 90% FBS + 10% DMSO
References: 1139: Gillis S, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. III. The isolation and phenotypic characterization of interleukin-2 producing T cell lymphomas. J. Immunol. 125: 2570-2578, 1980. PubMed: 6968790
23535: Mochizuki D, et al. Biochemical and biologic characterization of lymphocyte regulatory molecules. IV. Purification of Interleukin 2 from a murine T cell lymphoma. J. Immunol. 125: 2579-2583, 1980. PubMed: 7000903
