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平臺(tái)編號(hào)：bio-69047
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國際編號(hào)：HB-8068?
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細(xì)胞信息： 1D4
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
1D4
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：HB-8068?
數(shù)量：大量
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5mg Designations: 1D4
Depositors: ?The United States of America
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against nucleocapsid (p40) of herpes simplex virus type 1 (HSV-1)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:The original deposit of this cell line was contaminated with microorganisms. The line was treated with antibiotics and is now cured of the contamination. Animals were immunized with purified HSV-1 nucleocapsids.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody precipitates p40 and p45 of HSV-1 but not HSV-2.
The supernatant may have to be concentrated for use.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 1 mM sodium pyruvate and antibiotics, 15%; fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 70%; fetal bovine serum, 20%; DMSO, 10%
References: 1004: Zweig M, et al. Production of monoclonal antibodies against nucleocapsid proteins of Herpes simplex Virus types 1 and 2. J. Virol. 32: 676-678, 1979. PubMed: 91687
3510: Zweig M, et al. Test methods employing monoclonal antibodies against Herpes simplex virus types 1 and 2 nucleocapsids proteins. US Patent 4,430,437 dated Feb 7 1984
物種來源：小鼠
ATCC Number：HB-8068?
數(shù)量：大量
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5mg Designations: 1D4
Depositors: ?The United States of America
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against nucleocapsid (p40) of herpes simplex virus type 1 (HSV-1)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:The original deposit of this cell line was contaminated with microorganisms. The line was treated with antibiotics and is now cured of the contamination. Animals were immunized with purified HSV-1 nucleocapsids.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody precipitates p40 and p45 of HSV-1 but not HSV-2.
The supernatant may have to be concentrated for use.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 1 mM sodium pyruvate and antibiotics, 15%; fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 70%; fetal bovine serum, 20%; DMSO, 10%
References: 1004: Zweig M, et al. Production of monoclonal antibodies against nucleocapsid proteins of Herpes simplex Virus types 1 and 2. J. Virol. 32: 676-678, 1979. PubMed: 91687
3510: Zweig M, et al. Test methods employing monoclonal antibodies against Herpes simplex virus types 1 and 2 nucleocapsids proteins. US Patent 4,430,437 dated Feb 7 1984
