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平臺(tái)編號(hào)：bio-69028
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國(guó)際編號(hào)：CCL-135?
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細(xì)胞信息： LL 47 (MaDo)
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
LL 47 (MaDo)
ATCC Number：CCL-135?
細(xì)胞類(lèi)型：成纖維細(xì)胞
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
年限：16 years
器官來(lái)源：肺
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞形態(tài)：成纖維樣
數(shù)量：大量
規(guī)格：0.5mg Designations: LL 47 (MaDo)
Depositors: ?RG Crystal
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: lung
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: November, 1975
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 7,12
D13S317: 9,11
D16S539: 11
D5S818: 12,13
D7S820: 8,10
THO1: 7,9.3
TPOX: 9,10
vWA: 14,16
Cytogenetic Analysis:normal human male; diploid
At passage 9, the stemline number was diploid with a stable karyotype. One chromatid break was noted in 50 metaphase cells. The karyotype contained a brightly fluorescent Y chromosome as revealed by the quinacrine mustard fluorescence technique. Giemsa banding indicated no cross-contamination.
Isoenzymes: G6PD, B
Age: 16 years
Gender: male
Ethnicity: Black
Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%
Subculturing: Protocol: Remove medium. Add fresh 0.25% trypsin, 0.03% EDTA for 1 to 2 minutes. Remove the trypsin and incubate the culture 37C for 10 to 20 minutes with a small volume of trypsin, EDTA. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
細(xì)胞類(lèi)型：成纖維細(xì)胞
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
年限：16 years
器官來(lái)源：肺
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞形態(tài)：成纖維樣
數(shù)量：大量
規(guī)格：0.5mg Designations: LL 47 (MaDo)
Depositors: ?RG Crystal
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: lung
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: November, 1975
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 7,12
D13S317: 9,11
D16S539: 11
D5S818: 12,13
D7S820: 8,10
THO1: 7,9.3
TPOX: 9,10
vWA: 14,16
Cytogenetic Analysis:normal human male; diploid
At passage 9, the stemline number was diploid with a stable karyotype. One chromatid break was noted in 50 metaphase cells. The karyotype contained a brightly fluorescent Y chromosome as revealed by the quinacrine mustard fluorescence technique. Giemsa banding indicated no cross-contamination.
Isoenzymes: G6PD, B
Age: 16 years
Gender: male
Ethnicity: Black
Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%
Subculturing: Protocol: Remove medium. Add fresh 0.25% trypsin, 0.03% EDTA for 1 to 2 minutes. Remove the trypsin and incubate the culture 37C for 10 to 20 minutes with a small volume of trypsin, EDTA. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
