運輸方式：凍存運輸
是否是腫瘤細胞：0
物種來源：褐鼠
生長狀態(tài)：貼壁生長
品系：Harlan-Wistar
ATCC Number：CCL-38?
相關疾病：其他疾病
細胞形態(tài)：上皮樣
數(shù)量：大量
規(guī)格：0.5mg Designations: LLC-WRC 256
Depositors: ?RN Hull
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus
Morphology:epithelial
Source: Disease: mammary gland; Walker carcinoma
Strain: Harlan-Wistar
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 1
Tumorigenic:Yes
Propagation: ATCC complete growth medium: Medium 199, 95%; horse serum, 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22310: . . Anat. Rec. 124: 490, 1956.
26193: . . Science 117: 223, 1953.
