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THB-5 [HB-5, HB5]
    THB-5 [HB-5, HB5]
  • 平臺編號:bio-68987
  • 國際編號:HB-135?
  • 細(xì)胞信息: THB-5 [HB-5, HB5]
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

數(shù)量:大量
是否是腫瘤細(xì)胞:0
物種來源:小鼠
運(yùn)輸方式:凍存運(yùn)輸
生長狀態(tài):懸浮生長
細(xì)胞形態(tài):淋巴樣
免疫類型:IgG2a; kappa light chain
ATCC Number:HB-135?
規(guī)格:0.5mg Designations: THB-5 [HB-5, HB5]
Depositors: ?TF Tedder
Isotype: IgG2a; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human C3d receptor (CR2, Epstein-Barr virus (EBV) receptor); against CD21
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the SB human B cell line.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with the human C3d receptor (14500 dalton). This receptor also is the receptor for Epstein-Barr virus (EBV) on human B cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 990: Weis JJ, et al. Identification of a 145,000 Mr membrane protein as the C3d receptor (CR2) of human B lymphocytes. Proc. Natl. Acad. Sci. USA 81: 881-885, 1984. PubMed: 6230668

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