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平臺編號：bio-68976
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國際編號：HB-122?
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細(xì)胞信息： GAP A3
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
GAP A3
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2a; kappa light chain
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
ATCC Number：HB-122?
數(shù)量：大量
規(guī)格：0.5mg Designations: GAP A3
Depositors: ?AE Berger
Isotype: IgG2a; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against HLA A3
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human peripheral blood lymphocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody does not react with HLA A11.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 1 X 10(5) viable cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
Cell culture tested DMSO:ATCC 4-X
References: 22280: . . Fed. Proc. 42: 1371, 1983.
58007: Dieckmann CL, et al. Monoclonal antibody to HLA-A3. Hybridoma 1: 87-90, 1982. PubMed: 6208124
免疫類型：IgG2a; kappa light chain
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
ATCC Number：HB-122?
數(shù)量：大量
規(guī)格：0.5mg Designations: GAP A3
Depositors: ?AE Berger
Isotype: IgG2a; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against HLA A3
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human peripheral blood lymphocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody does not react with HLA A11.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 1 X 10(5) viable cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
Cell culture tested DMSO:ATCC 4-X
References: 22280: . . Fed. Proc. 42: 1371, 1983.
58007: Dieckmann CL, et al. Monoclonal antibody to HLA-A3. Hybridoma 1: 87-90, 1982. PubMed: 6208124
