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細(xì)胞類型：淋巴母細(xì)胞
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：人
生長狀態(tài)：懸浮生長
細(xì)胞形態(tài)：淋巴樣
ATCC Number：CRL-8015?
相關(guān)疾?。浩渌膊?br /> 運輸方式：凍存運輸
器官來源：脾
年限：5 years
規(guī)格：0.5mg Designations: TK6
Depositors: ?Massachusetts Institute of Technology
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast


Source: Organ: spleen
Disease: hereditary spherocytosis
Cell Type: lymphoblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
Age: 5 years
Gender: male
Comments:This line is derivative of the WIL-2 cell line.
The cells are heterozygous at the thymidine kinase (TK) locus, and can be
used to quantitatively detect forward mutation at 3 loci (resistance to trifluorothymidine (tk locus).
Resistance to thioguanine (hprt locus), and resistance to ouabain (Na/K ATPase].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0℃
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days (or as necessary to maintain the cell concentration between 2 X 10(5) and 1 X 10(6) cells/ml)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
WIL2-NS, ATCC CRL-8155
WIL2-S, ATCC CRL-8885
recommended serum:ATCC 30-2020
LTR228 , ATCC HB-8502
References: 4000: Skopek TR, et al. Assay for mutagenesis in heterozygous diploid human lymphoblasts. US Patent 4,302,535 dated Nov 24 1981
22402: Skopek TR, et al. Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay. Biochem. Biophys. Res. Commun. 84: 411-416, 1978. PubMed: 214074
22789: Levy JA, et al. Human lymphoblastoid lines from lymph node and spleen. Cancer 22: 517-524, 1968. PubMed: 5673231
32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642