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年限：11 to 13 days 

數(shù)量：大量 

運(yùn)輸方式：凍存運(yùn)輸 

ATCC Number：CRL-1715 

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器官來源：睪丸 

細(xì)胞形態(tài)：上皮樣 

生長(zhǎng)狀態(tài)：貼壁生長(zhǎng) 

細(xì)胞類型：其他細(xì)胞類型 

是否是腫瘤細(xì)胞：0 

物種來源：小鼠 

規(guī)格：0.5mg                                                                                                             Designations 菌種別名 : TM4 

Depositors:JP Mather    

Biosafety Level 生物安全等級(jí) :1   

Shipped 提供形式 :frozen 

Medium & Serum: See Propagation 

Growth  Properties:adherent

Organism:Mus musculus         

Morphology:epithelial       

Source:          

Organ: testis         

Disease: normal         

Cell Type:  

Sertoli cell;         

Cellular Products:retinol binding protein tissue plasminogen activator transferrin         

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits  may be required for the       transfer of this ATCC material. 

Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.          

Please click here  for information regarding the specific requirements for shipment to your location.             

Applications:transfection host         

Receptors:follicle stimulating hormone (FSH),expressed androgen receptor, expressed progesterone receptor, expressed       Tumorigenic:No         

Antigen        

Expression:H-Y antigen; Mus musculus, expressed         

Age:11 to 13 days         

Gender:male         

Comments 注釋 :The TM4 cell line is reported to respond to FSH with an increase in cAMP production, but to not respond to luteinizing hormone (LH). 

The FSH responsiveness is much reduced compared to primary sertoli cell cultures.

Constitutive plasminogen activator production is reported to be low, but is stimulated by FSH and, to a greater extent, by retinoic acid. Tested and found negative for ectromelia virus (mousepox).  

Propagation: ATCC complete growth medium:  A 1:1 mixture of Ham'S F12 medium and Dulbecco's modified Eagle's medium          with 1.2 g/L sodium bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5% 

Atmosphere 需氧情況 : air, 95%; carbon dioxide (CO2),5% 

Temperature 培養(yǎng)溫度 : 37.0°C         

Subculturing:   

Protocol:Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. 

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell           layer is dispersed (usually within 5 to 15 minutes). 

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. 

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 

Add appropriate aliquots of the cell supension to new culture vessels. Incubate cultures at 37C.         

Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:50 is recommended 

Medium Renewal: Every 3 to 4 days 

Preservation:  

Freeze medium: Culture medium, 95%; DMSO, 5%         

Storage temperature: liquid nitrogen vapor phase         

Related        

Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 recommended serum:ATCC 30-2040         

References 參考文獻(xiàn) : 1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781 

1159: Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium.Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561

1184: Carson DD, et al. Synthesis and secretion of a novel binding protein for retinol by a cell line derived from Sertoli cells.  J. Biol. Chem. 259:3117-3123, 1984. PubMed: 6538197 

26150: Mather JP, Phillips DM.Establishment of a peritubular myoid-like cell line and interactions between established testicular cell lines in culture.J. Ultrastruct. Res. 87: 263-274, 1984. PubMed: 6544874