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ATCC Number：HB-8502?
數(shù)量：大量
相關(guān)疾?。浩渌膊?br /> 生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：脾
年限：5 years
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgM; kappa light chain
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
規(guī)格：0.5mg Designations: LTR228 [LTR 228-TG, derivative of WIL-2NS (ATCC CRL-8155)]
Depositors: ?Cetus Corp.
Isotype: IgM; kappa light chain
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast


Source: Organ: spleen
Disease: hereditary spherocytosis
Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed
Cellular Products:immunoglobulin; monoclonal antibody (small amounts)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:The cells are resistant to 6-thioguanine and can be used in fusions with human B lymphocytes to produce human hybridomas.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
Cytogenetic Analysis:modal number = 48; Y present
Age: 5 years
Gender: male
Ethnicity: Caucasian
Comments:The cells are resistant to 6-thioguanine and can be used in fusions with human B lymphocytes to produce human hybridomas.
The cells have a high cloning efficiency in soft agar and by limiting dilution.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 0.03% L-glutamine and 0.02 mM 6-thioguanine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: fetal bovine serum, 95%; DMSO, 5%
Doubling Time: 16 hrs
Related Products:WIL2-NS, ATCC CRL-8155
TK6, ATCC CRL-8015
WIL2-S, ATCC CRL-8885
References: 3570: Raubitschek AR, et al. Human lymphoblastoid cell line and hybridomas derived therefrom. US Patent 4,624,921 dated Nov 25 1986